Researchers discover enzyme prototype for formation of ecologically and pharmaceutically important tropone compounds.

Previously unknown enzyme type

Bacteria found in terrestrial and marine environments produce tropone natural products, among other things, when they interact symbiotically with plants, algae or lower animals, for example as presumed protective substances against microbial pathogens in corals and sponges. The Freiburg researchers now investigated how the symbiotic bacteria produce these bioactive agents. Teufel and his team discovered a completely new type of enzyme that is essential for the production of these bacterial tropones.

Key intermediate in tropone biosynthesis

The scientists found that this enzyme activates oxygen in a previously unknown way and incorporates it into a chemical precursor compound. In the process, the basic structure of the tropone is generated. Using chemical and biochemical methods, the researchers were able to investigate the functions of this enzyme in more detail and thereby elucidate novel intermediates in tropone biosynthesis. «We succeeded in taking a crucial step toward better understanding the biological production of these significant compounds,» Teufel explains. «These findings can serve as a basis for better combating certain pathogens in the future or for obtaining novel tropone compounds using biotechnological methods.»

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